http://www.theolligist.com/blog daily 0.75 2024-03-12 http://www.theolligist.com/blog/2024/3/11/to-live-and-die-in-la-contrasting-life-and-death-of-two-distinct-algal-blooms-in-santa-monica-bay monthly 0.5 2024-03-13 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/c58a3c69-c3ed-4bd2-8e2d-a5a954b8d2e7/BloomMashup.png Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Algal blooms in lakes and coastal ecosystems. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/d30b2969-b33f-494c-aca8-c5c8f58ad3e1/upwelling_diagram.png Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Strong wind gusts moving south along the California coast during spring months results in the offshore transport of nutrient depleted surface water, and the upwelling of nutrient rich bottom water into the sun-lit layers where phytoplankton live in ample sunlight, poised to respond to the nutrient pulse. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/91c1f0c6-e7aa-4486-8363-9b96d58e2eaa/offtheblock.jpg Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Usain Bolt is a fast runner but he is equally fast off the starting block. Diatoms are like this. The respond quickly to nutrients and also grow quickly because of their surface area and genetically encoded strategies for nutrient utilization. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/14621a30-ba79-42ab-845f-4faf82c6e3d5/Slimdown.jpeg Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Like protein and carbohydrates, excess fat can be broken down and utilized when food consumption is not possible. Chitin is a nitrogenous compound found in some diatoms that may act as a nutrition storage molecule analogous to body fat. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/5b80adab-83b5-4653-8685-a0522f7e3ce8/food.jpeg Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Weird food. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/ab3226b5-303f-4b5a-92a9-71d216d24bbb/Figure2_nmds.png Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Non-metric, multi-dimensional scaling plot illustrating the groupings of Diatom (Grey=Pseudo-nitzschia, LakerGold=Thalassiosira) vs Dinoflagellate (LakerPurple) metabolic activity through both blooms (Diatom bloom in 2018=circles; Dino bloom in 2019=diamonds). Diatoms form a grouping separate from dinoflagellates which illustrates metabolic distinction, distinction in utilization of different strategies from their metabolic play book. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/e838af76-e10f-4f6a-a835-01009c9def04/Figure4.png Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Differential expression (mean log2 fold change relative to Day 1) of 36 functional categories grouped into four broad physiological processes of numerically dominant diatoms (A) vs. dinoflagellates (B) during periods (x-axis) of their bloom in 2018 vs. 2019, respectively. Nitrate + nitrite (Nitrogen), phosphate, and chlorophyll concentrations are shown in the top three rows. The four physiological processes are color-coded in the left-hand vertical column. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/72dda533-b3cd-4d32-9021-fee3072dd69b/AlternativeSplicing.png Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Alternatively spliced genes can produce multiple end products with distinct fates in the cell. In this example, segment B is retained or disgarded and results in differences in the subsequent protein product. If segment B contained a stop codon, failing to splice it out would result in the destruction of this transcript. This mode of gene regulation by alternatively spliced “Poison exons” is called nonsense-mediated decay (Lareau et. al. 2007). https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/57a9a475-21c8-4a92-90ab-54dff43b69a5/Footer.jpg Blog - To Live and Die in L.A.: Contrasting life and death of two distinct algal blooms in Santa Monica Bay - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.theolligist.com/blog/2024/2/2/examining-a-flavor-of-black-progress-through-the-lens-of-education monthly 0.5 2024-02-05 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/f5d12953-de83-49fe-a4ff-32f0ea82000e/BlackTotal.png Blog - Examining a flavor of "progress" through the lens of education in the USA - Make it stand out The fraction of degrees earned by people in the Black category of the Integrated Post-secondary Education Data System across an eleven-year time series. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/220b1614-91fd-403a-8ee1-5af3d4b6b0eb/BlackStem.png Blog - Examining a flavor of "progress" through the lens of education in the USA - Make it stand out The fraction of degrees in a STEM category (classified by the Department of Education) earned by people in the Black category of the Integrated Post-secondary Education Data System across an eleven-year time series. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/818811dc-aad9-4e3b-9856-788bebbfaf5d/Footer.jpg Blog - Examining a flavor of "progress" through the lens of education in the USA - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.theolligist.com/blog/2023/12/24/talking-out-of-school-the-racial-diversity-of-stem-degree-attainment monthly 0.5 2024-01-03 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/44c606ae-8a47-41f6-b68d-38715b0d5597/DivStatementFinal.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/77530f08-ac72-48e2-81aa-5156d7605a3b/MW_AWlevels.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Male vs Female degree attainment in 2019—all types, not just stem—at 5 award levels. X axis represents raw numbers in millions, Males represented by grey bars, Females, steel blue bars. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/9259fe4f-4e82-4b02-a654-6b28016e5fe7/StackedBar_CarnegieDemographics.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out An ugly figure that shows the raw data, the number of degrees earned and the relative proportions of all IPEDS racial categories at all award levels, including those that did not result in a degree. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/8869dc8f-aa9d-4f30-bb1b-8434dc8088b9/Americans_PCAvsNMDS.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Two methods of ordination of racial categories of U.S. citizens based on degrees at different levels (associates, bachelors, masters, PhD, Doctor), Majors (ie CIP families; “computer science”, “agriculture”, etc.) and institution type (ie carnegie classification; associates college, bachelorette college, research intensive college, etc.). Principle Component Analysis (Euclidean distance) is shown on the left; Non-metric Multi-dimensional scaling (Bray-Curtis dissimilarity) on the right. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/4b9f5466-cacb-4672-afdd-71f1fb08329d/ClusterDendrograms.jpg Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/e7f1c612-7ccc-4a46-9d99-037cc9a7b65b/Blackfrac_PhDbars.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/511521f4-3635-45fe-8438-d95899bfde8b/Blackfrac_Mastersbar.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/c88a7ded-f271-47ef-bf02-b49ff34c4ee2/Blackfrac_BS_bars.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/aa7d745c-d496-4918-b01d-e48bbf2590b8/Blkfrac_associatesbar.png Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Black American degrees: The fraction of degrees earned by Black American males and females at four degree levels across majors (CIP families). https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/66086429-2136-46b8-9506-caa241361650/Footer.jpg Blog - Talking Out of School: The Racial Diversity of STEM Degree Attainment - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.theolligist.com/blog/2023/12/12/building-names-have-been-changed-but-thats-about-it monthly 0.5 2023-12-12 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/14e50e17-cfc1-46d8-83e8-a74f46344583/milikan.jpeg Blog - Building names have been changed, but thats about it. - Make it stand out —Robert A. Millikan; source LA Times https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/367e07a5-b8b8-4801-a40c-887ba7b1ac2e/Caltech_bWO.jpg Blog - Building names have been changed, but thats about it. - Make it stand out If the graduating class of 2019 at Caltech was 100 students. There were 229 degrees awarded at Caltech in 2018. This figure illustrates the relative proportion of black (pompeian red) white (steel blue) and students with other (light grey) racial demographics. Source: https://nces.ed.gov/ipeds/use-the-data. http://www.theolligist.com/blog/2023/12/11/paper-number-two monthly 0.5 2023-12-13 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/81b21fb5-f96c-45d2-9615-1cf8ddc45a3d/Pier_cropped.jpg Blog - Daily dynamics of contrasting spring blooms on the California Coast - Make it stand out The site of much of my thesis project: The Santa Monica Pier, and the beautiful Southern California Coast. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/c6fc0d18-24cb-455e-b081-a22b63853690/Figure_S1.png Blog - Daily dynamics of contrasting spring blooms on the California Coast - Make it stand out April wind speed in Santa Monica Bay in 2018 (A) and 2019 (B). Vertical dashed lines indicate the start of sampling during each year. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1633b632-c50c-4a00-a65b-f59ad0e7b1fc/Figure_3.png Blog - Daily dynamics of contrasting spring blooms on the California Coast - Make it stand out Changes in the relative proportion of major protistan taxonomic groupings during the sampling periods in 2018 (A) and 2019 (B). https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/bc9a0700-a754-4f1f-9abf-c2a6c3b9d0ca/Figure_S4.png Blog - Daily dynamics of contrasting spring blooms on the California Coast - Make it stand out Images taken of the dominant protistan taxa from the 2018 and 2019 blooms via light microscopy. Black scale bar = 20 µm. A) Cryothecomonas sp. (Cercozoa) infecting Guinardia sp., B) Pseudo-nitzschia sp., C) Rhizosolenia sp., D) Akashiwo sp., E) Thalassiosira eccentrica, F) Margalefidinium fulvenscens http://www.theolligist.com/blog/2021/11/18/planting-sweetgrass-viewing-earth-as-a-gift-not-a-commodity-in-a-modern-world monthly 0.5 2021-11-18 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/f3eb6b93-4dfe-4370-b0f8-bb257e91c028/PotawatomiCamp.jpg Blog - Planting Sweetgrass: Viewing earth as a gift, not a commodity, in a modern world. - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.theolligist.com/blog/2021/8/4/lab-day-culture-check monthly 0.5 2021-08-13 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628105244824-EM1AJWTON2V4UPH9VXJ6/Margalefidinium.png Blog - Field notes from the "Live Area" and "Scope Room" - Make it stand out Chain of two Margalefidinium fulvescens under the scope. This critter is the subject of a quarter of my research. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628105146697-KGEX2LEZ5RPSN8B68F80/Acanth.png Blog - Field notes from the "Live Area" and "Scope Room" - Make it stand out Acantharia is a group of large heterotrophic protists. They were made famous through illustrations by Ernst Haeckel in the 1800s. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628105229213-IACBC0ZKE545ZVGJMYGX/Akashiwo.png Blog - Field notes from the "Live Area" and "Scope Room" - Make it stand out Akashiwo is a genus of dinoflagellate alga. It’s not known to be toxic, but can form some massive blooms with a brownish hue. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628105272990-51M0WOMCMITRNRIJNKYW/ceratium.png Blog - Field notes from the "Live Area" and "Scope Room" - Make it stand out Ceratium is an important photosynthetic dinoflagellate genus that is endemic to the California coast and present throughout the global ocean microbiome. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628105376279-NO7HX9H7RV4NI9UX1H9B/Coleps.png Blog - Field notes from the "Live Area" and "Scope Room" - Make it stand out Coleps is a heterotrophic protist (protozoa) that earns a living attaching to its prey and sucking out the contents. The contents here is the red circle. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628102080092-FLX3E00VJR5N4KACC88N/Footer.jpg Blog - Field notes from the "Live Area" and "Scope Room" - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.theolligist.com/blog/2021/2/20/my-first-paper monthly 0.5 2021-03-02 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1614459562292-IGY2C1UJ6YFLDALKZ908/GraphicalAbstract.png Blog - I Published My First Paper https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1614583900238-AL0EY6R0RAMUFOLKVSL0/Map-of-the-North-Pacific-Ocean-showing-the-North-Pacific-Subtropical-Gyre-and-the.png Blog - I Published My First Paper Satellite imagery illustrating the NPSG and station ALOHA. Bright green represents chlorophyll a, a proxy for photosynthetic activity. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1614548907688-E8L27GIHG5UNK6TWZ5R1/Footer.jpg Blog - I Published My First Paper http://www.theolligist.com/blog/2020/12/24/how-the-phyzer-vaccine-works-and-how-its-different-from-other-approaches monthly 0.5 2021-01-25 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1611511688740-ETM2C2G5I1AY9LRPMSL0/headerabstract.jpg Blog - "We Talkin 'bout Practice": How Pfizer, Moderna, and Sinovac vaccines work https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1611511187879-70PS7Y1BAUZIKA9VU4IJ/1_gQGV5yfkEcSi9XV7R_AABg.jpg Blog - "We Talkin 'bout Practice": How Pfizer, Moderna, and Sinovac vaccines work Anatomy of coronavirus https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1611511605147-3EWCCKDO4N1S79AX9NSF/Footer.jpg Blog - "We Talkin 'bout Practice": How Pfizer, Moderna, and Sinovac vaccines work http://www.theolligist.com/blog/2020/9/12/whats-the-meaning-of-success-from-an-ecological-network-perspective monthly 0.5 2020-12-21 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1607098166907-V4P8Z2D5WTF7ECXFZWYU/criminal_network-breakingbad2.jpg Blog - Its a small world: Network analysis of microbial communities Image 1. Crime boss reveled through network analysis. This is a criminal network from the show, “Breaking Bad” showing the known entities (photographs) and their associations (lines). If you watched the show, you know that Gustavo Fring (middle photo) was the mastermind behind a large methamphetamine enterprise. This network illustrates this role -Gustavo is the highest connected individual in the network. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1607271654149-VI8F0BIB37L1B8OTF054/social+network.jpeg Blog - Its a small world: Network analysis of microbial communities Image 2: Hypothetical social/professional network. The woman in the center has the most connections and may be very important. Note that everyone in the network is no more than just 1 person away from meeting her. Its a small world isn’t it? https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1607700293194-3REMT6QZW6BC67OIEMB6/Figure3.png Blog - Its a small world: Network analysis of microbial communities Image 3: Vertical distribution of protistan groups in the North Pacific Ocean (NPSG). There are a diversity of preferences, mostly based on sunlight, which does not penetrate beyond 175m (dotted line). https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1608394655465-P70T9IE9GF91PWJY7LNJ/image-asset.png Blog - Its a small world: Network analysis of microbial communities Image 4: Protistan interaction network from 5m to 770m. Circles represent an individual species, the size of the circle represents the number of interactions (lines) it has with other species. Circle colors represent the depths the species was found in abundance. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1608566418645-OE3V7UBQTHVJ728MJCWQ/Footer.jpg Blog - Its a small world: Network analysis of microbial communities http://www.theolligist.com/blog/2020/7/16/liquid-nitrogen-and-the-trans-atlantic-slave-trade monthly 0.5 2022-11-08 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1604250745722-4VDNW8W3XP2DD9NDG82R/LiquidNitrogen-slaveship.jpg Blog - Liquid Nitrogen and the Trans-Atlantic Slave Trade https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1604074986285-DC0U2G8QEEI26IGKT0DZ/microfly+copy.jpg Blog - Liquid Nitrogen and the Trans-Atlantic Slave Trade http://www.theolligist.com/blog/2020/10/15/happy-birthday-fela-kuti monthly 0.5 2020-10-15 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1602779658221-SGWWSS3MG22P7A113GMM/image-asset.jpeg Blog - Happy Birthday, Fela Kuti http://www.theolligist.com/blog/2020/9/21/photo-shoot-for-an-unknown-large-ciliate-taken-from-the-san-pedro-bay monthly 0.5 2020-10-04 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1600698558419-K5TK1QOU3DKW3R8W2IAH/Image_1228.jpg Blog - Photo shoot for an unknown large ciliate taken from the San Pedro Bay https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1600701547004-LD7XRLRUGVFFPU62KJ87/microfly+copy.jpg Blog - Photo shoot for an unknown large ciliate taken from the San Pedro Bay http://www.theolligist.com/blog/2020/8/16/the-red-tide-sn5tc monthly 0.5 2020-09-14 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1599667556853-S6N3G6W4DY5IJHYL2SDU/image-asset.jpeg Blog - The demise of an algal dynasty: Red Tide April 2020 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1599667529447-U74ROUIDWN2U9GSSESIK/TDB-L-REDTIDE-0910-277.jpg Blog - The demise of an algal dynasty: Red Tide April 2020 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1600018249477-9LVCXH3C2MMUWAE8JUZ1/33-4-1-Coleps-amphacanthus.jpg Blog - The demise of an algal dynasty: Red Tide April 2020 Artist rendition of a heterotrophic protist (coleps) filled with and digesting grazed algal prey. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1600018483146-ZATMSUREVD2YDRH7W8JQ/Noct_blog.jpg Blog - The demise of an algal dynasty: Red Tide April 2020 Noctiluca collected from the San Pedro Bay, June 2020. Nodules the shape of Lingulodinium can be seen inside of Noctiluca. A free-living Lingulodinium cell can also be seen in bottom right corner of the rightmost photograph. http://www.theolligist.com/blog/2020/8/7/the-new-lab monthly 0.5 2020-09-12 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1597503993160-VLFF8JU2G6BP4HF56MST/microbiome_research_covers.jpg Blog - Thank marine microbes for every other breath you breathe. Magazine covers from 2012 illustrating just how important discoveries coming from the Human Microbiome Project https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598457358507-6X58D97NJ0EE78RKKZ2Z/phytoplankton-Diatoms-ocean-food-chains-foundations-fishes.jpg Blog - Thank marine microbes for every other breath you breathe. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598468582169-ORX3Z0DBADYQ2PU8PRP9/microfly+copy.jpg Blog - Thank marine microbes for every other breath you breathe. http://www.theolligist.com/blog/2018/8/16/the-community-will-never-be-the-same monthly 0.5 2020-08-25 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1594655390473-VPFPWU85RSL3L1KSQT93/westcoast-top.jpg Blog - Here today, gone three days later: Algal blooms on the Southern California Coast Algal bloom: Satellite imagery of chlorophyll a concentrations just north of the Los Angeles metropolitan area. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596475658620-JHRUW8RKSSCT5QNAGA2Y/phytoplankton-Diatoms-ocean-food-chains-foundations-fishes.jpg Blog - Here today, gone three days later: Algal blooms on the Southern California Coast Simplified marine food chain starts with primary producers, photosynthetic organisms and works itself up the chain to larger and larger organisms. The whole chain breaks down if we remove any link, especially the primary producers, which supply the entire system. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596477943260-2KXWT6IHUBL4VEWEUG89/Algae_mashup.png Blog - Here today, gone three days later: Algal blooms on the Southern California Coast Flavors of algae: Shown are just a few common genera of algae with their own evolutionary history, skills, preferences, requirements, tolerances, and metabolic capacities. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596479361230-WF1BWA0X1MHLSHT0MBXY/avp.jpg Blog - Here today, gone three days later: Algal blooms on the Southern California Coast Characteristics of two notorious algae on the California coast. Alexandrium and Pseudonitzschia are two genera of algae that have the ability to produce toxins. Pseudonitzschia, being the diatom has a large size, which probably gives it an advantage in high-nutrient, spring conditions, whereas the mobile and small Alexandrium most likely dominates in the calm, low nutrient summer waters. Despite its size, its can pack a punch. Saxitoxins can be extremely deadly. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596658414739-6EZ8FZPCKARJCDZAC3IT/Pier_cropped.jpg Blog - Here today, gone three days later: Algal blooms on the Southern California Coast My study site, The Santa Monica Bay (Santa Monica Pier) https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596658563581-SF11M3YD2WFQHC9EA2QF/SM_Pier-logistics.jpg Blog - Here today, gone three days later: Algal blooms on the Southern California Coast Santa Monica Pier https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596387941008-1XBY2D1LTWEZIN9R1EYX/AmpliconSequencing.png Blog - Here today, gone three days later: Algal blooms on the Southern California Coast From seawater to microbial community structure. Water is collected and (1) passed through a special filter while things larger than the pore size of 0.7µm, ie most protists, are captured onto it. (2) DNA and RNA are extracted sequenced, and (3) the differences are interpreted and designated as distinct variants (‘operational taxonomic units (OTUs) or amplicon sequence variants (ASVs)) using computer algorithms and later given species names by running those sequences through a database containing similar sequences and the names of the organisms from which the sequence originated. (4) finally, the ASVs or OTUs are tallied to give an overall picture of the relative proportion of each species in the community and facilitate more rigorous statistical analyses. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596480732567-V5HQNO1N895TMVA7RO4G/cascade.png Blog - Here today, gone three days later: Algal blooms on the Southern California Coast The chain reaction: Cascade of events from wind driven upwelling of Nitrogen and phosphorus and the rapid proliferation of algae as a result (chlorophyll a concentration). https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596481026808-9C69XY7CIPNJRJSOBTPM/Diatom-DinoDiversity.png Blog - Here today, gone three days later: Algal blooms on the Southern California Coast Daily dynamics in the diatom and dinoflagellate communities according to 18S rRNA sequencing. Only a few of several species proliferate by outcompeting the others for limited nutrients (Nitrogen and Phosphorous). https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598392720991-V5LCHG5J07BSGWDK2E2U/microfly+copy.jpg Blog - Here today, gone three days later: Algal blooms on the Southern California Coast http://www.theolligist.com/blog/2015/9/4/the-climb monthly 0.5 2017-08-09 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1497638366290-ZVQKD39TUXE2EEZNDANX/image-asset.jpeg Blog - The evolution of distinct complexity Dscam orthology: Greavily lab http://graveleylab.cam.uchc.edu/Graveley/ https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1497633319893-UMRG0V1QY4FGK9SPLR96/image-asset.jpeg Blog - The evolution of distinct complexity METAZOA Paps et al. 2009 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1497634143809-W5ZWDQE4DE7ZRWH1EWQH/image-asset.jpeg Blog - The evolution of distinct complexity Amoeba proteus http://www.realmonstrosities.com/2011/01/amoeba-proteus.html https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1502321047689-3JNR7PN6J3M8EHV5RYN3/image-asset.jpeg Blog - The evolution of distinct complexity http://www.theolligist.com/blog/2017/6/30/13-tattooed-tears-crispr-region-of-s-thermophilus monthly 0.5 2021-01-26 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1502148760786-AHW6L0KPZ1IPLTCUSZWR/image-asset.jpeg Blog - 13 Tattooed Tears: CRISPR region of S. thermophilus Tattoo Tears (Prison Context) https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1502149113861-KFUZE93H5LVYYHUP8P87/image-asset.jpeg Blog - 13 Tattooed Tears: CRISPR region of S. thermophilus Electron micrograph of S. Thermophilus colony https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1498851327609-262F46S36W8E56VY9ABU/image-asset.jpeg Blog - 13 Tattooed Tears: CRISPR region of S. thermophilus Linear representation of the genome of S. thermophilus. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1498851353426-HM5WH8IDBJ5968WU7QZB/image-asset.jpeg Blog - 13 Tattooed Tears: CRISPR region of S. thermophilus Zoom in view of the CRISPR region. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1498851384286-6CMRU8SQF4M601ADREYH/image-asset.jpeg Blog - 13 Tattooed Tears: CRISPR region of S. thermophilus Zoom in within the CRISPR region https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1498851684036-4D2Q8HM30D05461XE9GF/image-asset.jpeg Blog - 13 Tattooed Tears: CRISPR region of S. thermophilus https://www.nature.com/scitable/definition/bacteriophage-phage-293 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598392831157-17P63RHC3WX85HCI9RBS/microfly+copy.jpg Blog - 13 Tattooed Tears: CRISPR region of S. thermophilus http://www.theolligist.com/blog/2015/10/2/forced-labor-camp monthly 0.5 2020-09-19 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1488322865145-SHU7QVK25V42X6US7S5Z/image-asset.jpeg Blog - Forced Labor Camp The miniprep: One plasmid to billions https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1488322056190-MTSD8SIQIHD9BIM20UGN/Minipreplabeled.jpg Blog - Forced Labor Camp https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1488323391321-MKHS81TJ0XOHCK4F1KS0/microfly+copy.jpg Blog - Forced Labor Camp http://www.theolligist.com/blog/2015/9/29/hoodoo-of-gene monthly 0.5 2016-07-17 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1444006062134-2PMF2SDDDEF5RFUG8ULX/AS_deebo.jpg Blog - Introduction to Alternative Splicing: The "Street Gene" Hypothetical alternatively spliced "public Display gene." https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1444079471160-1Q6GV5CF7OLTPY7EDE5O/image-asset.jpeg Blog - Introduction to Alternative Splicing: The "Street Gene" Phenotypes of the Public Display gene https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1444342764121-WMK8UU3G1HSGGXXOTZN2/image-asset.jpeg Blog - Introduction to Alternative Splicing: The "Street Gene" Exons (colored boxes) are joined together after the removal of certain Introns (black lines) to produce a Transcript. In this case Transcript A. Transcript A, containing exons A, B & C, is then translated to produce a distinct protein; producing the Wayne Brady phenotype. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1444343004959-JUY7O1U21QQE6KKB8ZU1/image-asset.jpeg Blog - Introduction to Alternative Splicing: The "Street Gene" Under stressful conditions, the same public display gene produces a different transcript; Transcript B. Transcript B, containing only exons A & C, makes a distinct protein; producing the Deebo phenotype. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1444343250553-VPE3IO9DP7NA49GDKEV5/image-asset.jpeg Blog - Introduction to Alternative Splicing: The "Street Gene" Alternatively spliced public display gene. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1444366584235-CDSW8JMAZEZDR1ZHKNLU/image-asset.jpeg Blog - Introduction to Alternative Splicing: The "Street Gene"     https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1463002862588-ZG7V39PSEJ94DVP0KFA4/image-asset.jpeg Blog - Introduction to Alternative Splicing: The "Street Gene" Multi-dress: One dress, six distinct styles. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1467610896818-PPJLL5MBBBJ6GV0G4BAC/image-asset.jpeg Blog - Introduction to Alternative Splicing: The "Street Gene" http://www.theolligist.com/blog/2015/8/30/eejtxt monthly 0.5 2015-09-20 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1440922902877-JDDMCFVWCCDYXSRIFA1G/image-asset.jpeg Blog - The Lab golli $ more EEJ.txt https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1441415017393-FTRI250VW1KM3KFXJ4GN/image-asset.jpeg Blog - The Lab I enter the world https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1442703183439-QE9X5928A3X9WIS6P6PR/image-asset.jpeg Blog - The Lab https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1442702903401-MN2YHOM0O9A75CABTUQN/image-asset.jpeg Blog - The Lab https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1442704555911-V7JS7J6TSOF6PWP2F5WO/dna.jpg Blog - The Lab https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1442706753839-ECKVFDC9XUMXCSQZWV3G/microfly+copy.jpg Blog - The Lab http://www.theolligist.com/blog/2015/9/11/welcome monthly 0.5 2015-09-20 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1442003677575-71GYY3T1JIAS9QHM8KVU/biomarin Blog - Welcome BioMarin Pharmaceutical Inc. http://www.theolligist.com/alps-avenue daily 0.75 2025-05-09 https://images.squarespace-cdn.com/content/v1/4fd1028ee4b02be53c65dfb3/1437046033467-ERR9FSD1WLWIAU16PGMO/440d9cf2f085ab73b22318d48e6eea8ae3af3.jpg Mountains https://images.squarespace-cdn.com/content/v1/4fd1028ee4b02be53c65dfb3/1437046033467-ERR9FSD1WLWIAU16PGMO/440d9cf2f085ab73b22318d48e6eea8ae3af3.jpg Mountains https://images.squarespace-cdn.com/content/v1/4fd1028ee4b02be53c65dfb3/1377893609489-9E7JZJAV07MXXAN6VJ4F/65ce5b7508516e805fbb00f8d5ac1f97.jpg Mountains https://images.squarespace-cdn.com/content/v1/4fd1028ee4b02be53c65dfb3/1377893608271-HQWXY7OI2YI2EFF53RUH/72236f774a722fa8a08f5b886ab642e2.jpg Mountains https://images.squarespace-cdn.com/content/v1/4fd1028ee4b02be53c65dfb3/1377893608450-SXXQ7OHJWSKCGY2EPZZI/839847d4183a8ad591c87568a5d2cdcd.jpg Mountains 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coastline. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628832999861-01220KS8Y410X8S4XTQO/IMG_4630.jpg Gallery - Make it stand out Microcystis bloom, Clear Lake, CA https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628832852290-V1Z4NIIT6VLTP8523VYV/IMG_4670.jpg Gallery - Make it stand out Scooping a bowl of Microcystis soup. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1628831848210-226OCX40AJNVBIQT2M0G/Clear-15.jpg Gallery - Make it stand out Capturing Microcystis colonies in Clear Lake, CA https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1536467811575-CUYOS1VV0SY3HYGC4QTT/828A6073.JPG Gallery Shot off the pier @ The Wrigley Marine Institute, Catalina Island. RV Yellow fin shown in front of the town, Two Harbors. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598548754787-ALYPOFYXOT0GFTIW2QMU/website-10.jpg Gallery “Tale of Two Towns”: The Wrigley Institute Marine Science Center (right) and Two Harbors (left), Catalina Island https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598201937161-F693FPUJK8017YSFKBEY/PofWeekAug9.png Gallery The same welcome committee as we return from a day of sampling at the San Pedro Ocean Time Series. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598202113755-SBD35BRQ39Z6XBT2A1JJ/PofWeekjul5.png Gallery “The Egret”, somewhere on Catalina Island. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598202159680-KQ2A1EOOBYW6TEYGMUX1/Random-2.jpg Gallery “Gone Fishing”. At the end of this steel cable there are niskin bottles headed to specific depths to collect water. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1615650821555-E2S27V0ERUQ84FSYXH4E/angeles.jpeg Gallery Niskins return with water from the depths and the microbes that live there. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598202196396-BGVU146242Q10VV04VEC/Random-3.jpg Gallery “Sampling Dexterity”. Niskin bottles suspended in a rosette being drained after returning from the abyss. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598202350359-YLWPNJH32S40OP2VDV2C/ka-sunset-8.jpg Gallery View from the Kauaian coast. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1598202399661-OVLT9DD6H1BTDZP6SFOM/ka-sunset-7.jpg Gallery “Golden Sunset” color palate. https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1615650668431-0E2FOMGH31XGNP2N9W51/cranes.jpg Gallery Ewocks (Starwars) on the San Pedro Bay http://www.theolligist.com/contact daily 0.75 2015-09-18 http://www.theolligist.com/about daily 0.75 2024-04-30 https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/070ca269-1fbb-4e83-aaf2-ce1e3a837ce6/rgs-6.jpg About https://images.squarespace-cdn.com/content/v1/55e17b39e4b0569a25662501/1596732236568-GSJW8DSURBT8259WDFK7/Day1-13.jpg About Protists on the California coast: Typical characters you may find in every drop of water you swallow at the beach. Taken from H2O at the Santa Monica Pier. Protists in Figure A were shot on a vintage/cheap microscope in my closet so anyone can do it! http://www.theolligist.com/new-page daily 0.75 2016-08-26 http://www.theolligist.com/evolution-of-the-ls2-regulated-splicing-network daily 0.75 2016-08-26 http://www.theolligist.com/pagecv daily 0.75 2025-10-26